名    称: Epidermal Growth Factor Protein, Human recombinant
英文名称: Epidermal Growth Factor Protein, Human recombinant
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品    牌: Millipore
规    格: 500 μg
货    号: GF144
CAS   号: 暂无CAS号
保存环境: 数据暂无
区    域:
厂家报价: ¥2990.00元
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Cytokines and Growth Factors

EMD Millipore offers a comprehensive range of highly purified bioactive cytokines and growth factors for use in cell culture, stem cell differentiation and ELISA studies. We have cytokines and growth factors for human, mouse, and rat research, including human animal-free and human xeno-free material. These proteins and many of our related medias and assays that use these cytokines are based on the scientific expertise of Upstate & Chemicon. All have been well validated and published.

Human ES cell line, H9, derived neural progenitors were subjected to directed differentiation to dopaminergic neurons using SCR128. Cells were fixed and stained with neuronal marker, MAP2 (MAB3418) and dopaminergic neuron marker, Tyrosine Hydroxylase (AB152) at 1:400 dilution and counter stained with nuclei dye, DAPI.

 
 
Description:
Epidermal Growth Factor, human recombinant
 
Trade Name:
Chemicon (Millipore)
 
Qty/Pk:
500 μg
 
Product Overview:
Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Human EGF is a 6.2 kDa protein containing 53 amino acid residues.
 
Application Notes:
For most in vitro applications, EGF exerts its biological activity in the concentration range of 0.5 to 25.0 ng/mL. Responding cells are (partial list): Epidermal mesothelial cells.
 
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
 
Entrez Gene Summary:
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
 
UniProt Summary:
FUNCTION: SwissProt: P01133 # EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.
SIZE: 1207 amino acids; 133946 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P01133 ## Contains 9 EGF-like domains. & Contains 9 LDL-receptor class B repeats.
 
Brand Family:
Chemicon
 
Source:
E.coli
 
Presentation:
Lyophilized from sterile filtered 10 mM Sodium Phosphate, pH 7.5, containing no additives.
 
Storage Conditions:
The lyophilized EGF, though stable at room temperature, is best stored desiccated at -20°C. Reconstitute with water to a concentration of 0.1 - 1 mg/mL. This solution can be diluted into other buffered solutions and stored at 4°C for 1 week or -20°C for future use. Reconstituted EGF should be maintained at -20°C in undiluted aliquots for up to one year.
Replaces:
GF001
 
Gene Symbol:
  • EGF
  • URG
  • urogastrone
 
Product Name:
Epidermal Growth Factor, human recombinant
Alternate Names:
EGF
 
UniProt Number:
 
Specific Activity:
Human EGF is fully biologically active when compared to standards. The ED50, as determined by its dose-dependent mitogenic activity on BALB/c 3T3 cells, is ≤ 0.1 ng/mL, corresponding to a specific activity of ≥ 1 x 10^7 units/mg.
Entrez Gene Number:
 
Purity:
Greater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of EGF.

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