名    称: Immobilon Western Chemiluminescent HRP Substrate
英文名称: Immobilon Western Chemiluminescent HRP Substrate
原始链接: 点击查看
品    牌: Millipore
规    格: 100 ML
货    号: WBKLS0100
CAS   号: 暂无CAS号
保存环境: 2–8°C
区    域:
厂家报价: ¥902.00元
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For simple, cost-effective protein detection, use Millipore's Western blotting reagents to achieve the highest sensitivity with the least background.

Bløk™ Noise Cancelling Reagents

Bløk reagents minimize background in Western blots, increasing sensitivity for superior detection of even low-abundance proteins. Bløk reagents are protein-free, which reduces non-specific binding, especially when you are using phosphoantibodies or avidin-biotin systems.

While Bløk reagents were specially engineered for Millipore’s SNAP i.d. detection system, they can be used to reduce background in traditional Western blotting protocols.

Choose from 3 Bløk reagents

 

  • Bløk-CH buffer for HRP chemiluminescence or chromogenic detection
  • Bløk-FL buffer for fluorescence detection
  • Bløk-PO buffer for phosphotyrosine detection using chemiluminescence or fluorescence

 

Bløk Buffer Features:

 

  • Ready-to-use
  • Protein-free
  • Will pass freely through SNAP i.d. blot holders
  • Can be stored at room temperature
  • Long shelf life:

• Bløk-CH/FL 2 year shelf-life
• Bløk-PO 1 year shelf-life
  • Available in 500 mL bottle
  • Compatible with PVDF (Immobilon P, Immobilon FL) and nitrocellulose
  • Bløk-CH reagent compatible with Immobilon HRP substrate
  • Compatible with streptavidin antibodies
  • Ability to dilute the 1° and 2° antibodies using Bløk reagents
  • Sufficient reagent for 19 mini blots (each 8 x 7 cm blot requires at least 26 mL for SNAP i.d.)

 

Why use a synthetic blocker?

 

  • Reduce cross-reactivity which may be observed with protein-based buffers
  • Reduce background, especially for detecting low-abundance proteins
  • If you are studying protein phosphorylation
  • If you are using an avidin-biotin system

 

Simplify troubleshooting

Bløk reagents allow you to stain the membrane after immunodetection so you can see if there were problems with the protein transfer, or learn whether each lane contained the same amount of protein.

Luminata Western HRP Subtrates

The new family of Luminata Western HRP substrates are a family of premixed, ready-to-use chemiluminescent reagents for the detection of HRP-based Westerns. Pour directly onto the Western membrane without worrying about pipetting error. The Luminata™ Classico, Crescendo and Forte substrates cover a broad range of sensitivities and are stable at 4 °C or room temperature so you can store it on your own bench.

Immobilion® Western HRP and AP Chemiluminescent Substrates

Millipore’s HRP and AP substrates excel in all Western and dot/slot/spot blotting applications. In addition, they are compatible with both PVDF and nitrocellulose membranes, as well as all commonly used buffers and blocking reagents.

Spray and Glow Detection Reagent

The Visualizer™ Spray & Glow™ system is a light-emitting non-radioactive method for detecting immobilized specific antigens in chemiluminescent Western blots through HRP-labeled antibodies. Spray & Glow is comparable in sensitivity to an everyday “workhorse” ECL detection reagent, suitable for normal to high abundance proteins. As a plus, Spray & Glow is easy to use and requires no mixing or pipetting!

Its low background, high signal-to-noise ratio make it ideal for your research. As you may know, low background blots are considered higher quality data that are easier to analyze and more likely to be accepted for publication.

 

 

 

Detection of GAPDH in 10 μg (Lane 1), 5 μg (Lane 2), 2.5 μg (Lane 3), and 
1.2 μg (Lane 4) of A431 lysate. Blots were treated with the indicated detection 
substrate and exposed to x-ray film for 5 minutes. Luminata Forte substrate is 
equivalent to Immobilon Western Chemiluminescent HRP Substrate (WBKLS0500, 
Millipore).

Detection of GAPDH in 10 μg (Lane 1), 5 μg (Lane 2), 2.5 μg (Lane 3), and 1.2 μg (Lane 4) of A431 lysate. Blots were treated with the indicated detection substrate and exposed to x-ray film for 5 minutes. Luminata Forte substrate is equivalent to Immobilon Western Chemiluminescent HRP Substrate (WBKLS0500, Millipore).

说明:
Immobilon Western HRP 底物,2 x 50 mL
商标名:
Immobilon
数量/包装:
2 x 50 mL
应用:
Western blotting, dot/slot blotting
关键应用:
Western Blotting
 
膜覆盖度,cm2:
1000
 
保存温度,°C:
2–8
试剂(体积):
发光氨 (50 mL); 过氧化物溶液 (50 mL)
保存条件:
2–8
体积,mL:
100
产品名称:
Immobilon Western 化学发光 HRP 底物
过滤面积,cm2:
1000
 


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